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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
18/02/2022 |
Data da última atualização: |
25/02/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIEIRA, A. A. R.; SOUZA, L.C. de; SILVA JÚNIOR, A. L. da; ALVES, B. Q.; MIRANDA, F. D. de; MOREIRA, S. O.; CALDEIRA, M. V. W. |
Afiliação: |
Alessandra Abreu Rodrigues Vieira, UFES; Lucimara Cruz de Souza, UFES; Adelson Lemes da Silva Júnior, UFES; Bruno Quinelato Alves, UFES; Fábio Demolinari de Miranda, UFES; Sarah Ola Moreira, Incaper; Marcos Vinicius Winckler Caldeira, UFES. |
Título: |
Diversity and genetic structure of Astronium concinnum Schott ex Spreng. in conservation units. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Plant Genetic Resources: Characterization and Utilization, p. 1?8, 2022. |
DOI: |
10.1017/S1479262121000654 |
Idioma: |
Inglês |
Conteúdo: |
Astronium concinnum Schott ex Spreng. (Anacardiaceae) is a species used in civil construction, naval, luxury furniture, in addition to the potential for recovery and restoration of habitats. The objective of this work was to characterize the diversity and genetic structure of the A. concinnum in the Conservation Units, National Forest of Pacotuba and Private Natural Heritage Reserve of Cafundó, located in the south of the state of Espírito Santo. Eight ISSR primers were used, which produced 121 DNA fragments and 73.55% polymorphism. In the analysis of genetic dissimilarity, seven distinct groups were identified, with the majority of individuals (from both Conservation Units) being brought together into a single group. The genetic diversity of Nei (H*) and the Shannon index (I*), provided values for the species of 0.312 and 0.473, respectively, indicating the genetic diversity conserved in the species and its potential use for collecting genetically diversified seeds. The analysis of molecular variance revealed that most of the diversity (92.54%) is distributed within populations and the value of gene flow (Nm = 10.629) indicates the high rate of genetic exchange between Conservation Units. The results of the genetic structuring indicated the division of individuals into three genetic groups (K = 3), however, it was possible to observe a mixture of genetic material with the sharing of alleles between the three groups. The results indicate that A. concinnum trees maintain genetic diversity for their maintenance. In addition, the potential of the analysed individuals was certified as future matrixes for seed collection. MenosAstronium concinnum Schott ex Spreng. (Anacardiaceae) is a species used in civil construction, naval, luxury furniture, in addition to the potential for recovery and restoration of habitats. The objective of this work was to characterize the diversity and genetic structure of the A. concinnum in the Conservation Units, National Forest of Pacotuba and Private Natural Heritage Reserve of Cafundó, located in the south of the state of Espírito Santo. Eight ISSR primers were used, which produced 121 DNA fragments and 73.55% polymorphism. In the analysis of genetic dissimilarity, seven distinct groups were identified, with the majority of individuals (from both Conservation Units) being brought together into a single group. The genetic diversity of Nei (H*) and the Shannon index (I*), provided values for the species of 0.312 and 0.473, respectively, indicating the genetic diversity conserved in the species and its potential use for collecting genetically diversified seeds. The analysis of molecular variance revealed that most of the diversity (92.54%) is distributed within populations and the value of gene flow (Nm = 10.629) indicates the high rate of genetic exchange between Conservation Units. The results of the genetic structuring indicated the division of individuals into three genetic groups (K = 3), however, it was possible to observe a mixture of genetic material with the sharing of alleles between the three groups. The results indicate that A. concinnum trees maintain gene... Mostrar Tudo |
Palavras-Chave: |
Collecting seeds; Genetic variability; ISSR markers. |
Thesagro: |
Anacardiaceae. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4088/1/diversity-and-genetic-structure-of-astronium-concinnum-schott-moreira.pdf
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Marc: |
LEADER 02438naa a2200253 a 4500 001 1023793 005 2022-02-25 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1017/S1479262121000654$2DOI 100 1 $aVIEIRA, A. A. R. 245 $aDiversity and genetic structure of Astronium concinnum Schott ex Spreng. in conservation units.$h[electronic resource] 260 $c2022 520 $aAstronium concinnum Schott ex Spreng. (Anacardiaceae) is a species used in civil construction, naval, luxury furniture, in addition to the potential for recovery and restoration of habitats. The objective of this work was to characterize the diversity and genetic structure of the A. concinnum in the Conservation Units, National Forest of Pacotuba and Private Natural Heritage Reserve of Cafundó, located in the south of the state of Espírito Santo. Eight ISSR primers were used, which produced 121 DNA fragments and 73.55% polymorphism. In the analysis of genetic dissimilarity, seven distinct groups were identified, with the majority of individuals (from both Conservation Units) being brought together into a single group. The genetic diversity of Nei (H*) and the Shannon index (I*), provided values for the species of 0.312 and 0.473, respectively, indicating the genetic diversity conserved in the species and its potential use for collecting genetically diversified seeds. The analysis of molecular variance revealed that most of the diversity (92.54%) is distributed within populations and the value of gene flow (Nm = 10.629) indicates the high rate of genetic exchange between Conservation Units. The results of the genetic structuring indicated the division of individuals into three genetic groups (K = 3), however, it was possible to observe a mixture of genetic material with the sharing of alleles between the three groups. The results indicate that A. concinnum trees maintain genetic diversity for their maintenance. In addition, the potential of the analysed individuals was certified as future matrixes for seed collection. 650 $aAnacardiaceae 653 $aCollecting seeds 653 $aGenetic variability 653 $aISSR markers 700 1 $aSOUZA, L.C. de 700 1 $aSILVA JÚNIOR, A. L. da 700 1 $aALVES, B. Q. 700 1 $aMIRANDA, F. D. de 700 1 $aMOREIRA, S. O. 700 1 $aCALDEIRA, M. V. W. 773 $tPlant Genetic Resources: Characterization and Utilization, p. 1?8, 2022.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
08/01/2014 |
Data da última atualização: |
01/09/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
ABREU, P. M. V.; PICCIN, J. G.; RODRIGUES, S. P.; VENTURA, J. A.; FERNANDES, P. M. B. |
Afiliação: |
Paolla M.V. Abreu, UFES; João G. Piccin, UFES; Silas P. Rodrigues, UFES; Jose Aires Ventura, Incaper; Patricia M.B. Fernandes, UFES. |
Título: |
Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam-Holanda, v. 180, p. 11-17, 2012. |
Páginas: |
7p. |
ISSN: |
0166-0934 |
DOI: |
10.1016/j.jviromet.2011.12.003 |
Idioma: |
Português |
Conteúdo: |
Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. |
Palavras-Chave: |
DsRNA; Papaya; Papaya meleira virus; Phytopathology; Real-time RT-PCR; Resistance induction. |
Categoria do assunto: |
-- |
URL: |
http://ac.els-cdn.com/S0166093411004885/1-s2.0-S0166093411004885-main.pdf?_tid=597935f2-7857-11e3-a060-00000aacb35e&acdnat=1389180411_31ada3a608c8d17c0a26fd345cb062ae
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/162/1/JVirolMethods-2012.PDF
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Marc: |
LEADER 01746naa a2200277 a 4500 001 1001438 005 2015-09-01 008 2012 bl --- 0-- u #d 022 $a0166-0934 024 7 $a10.1016/j.jviromet.2011.12.003$2DOI 100 1 $aABREU, P. M. V. 245 $aMolecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.$h[electronic resource] 260 $c2012 300 $a7p. 520 $aPapaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. 653 $aDsRNA 653 $aPapaya 653 $aPapaya meleira virus 653 $aPhytopathology 653 $aReal-time RT-PCR 653 $aResistance induction 700 1 $aPICCIN, J. G. 700 1 $aRODRIGUES, S. P. 700 1 $aVENTURA, J. A. 700 1 $aFERNANDES, P. M. B. 773 $tJournal of Virological Methods, Amsterdam-Holanda$gv. 180, p. 11-17, 2012.
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